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The surname Masterton first appeared in 1296 when William de Mastertone, or de Meystertone, from Fife rendered homage for his possessions. A lion rampant with a rose in front and his name S' Will'i de Maisterton appear on his seal.

The Masterton family was one of note in the Dunfermline area before the Reformation. In 1316, a Duncan de Maysterton was witness to the homage of Duncan, Earl of Fife to the abbot of Dunfermline.
A Symon de Maysterton, a rector, was a witness in 1357, and Sir Thomas Masterton, in 1476, was a canon regular of Cambuskenneth.